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Literature summary extracted from

  • Wang, X.; Feng, J.; Xue, Y.; Guan, Z.; Zhang, D.; Liu, Z.; Gong, Z.; Wang, Q.; Huang, J.; Tang, C.; Zou, T.; Yin, P.
    Corrigendum Structural basis of N6-adenosine methylation by the METTL3-METTL14 complex (2017), Nature, 542, 260 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.348 the METTL3-METTL14 complex is co-expressed in High Five (Trichoplusia ni) insect cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.348 sitting drop vapor diffusion method, using 18% (w/v) PEG 8000 and 0.1 M sodium citrate, pH 5.7 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.348 D377A the METTL3 mutation completely abolishes enzyme activity Homo sapiens
2.1.1.348 D395A the METTL3 mutation completely abolishes enzyme activity Homo sapiens
2.1.1.348 E532A the METTL3 mutation completely abolishes enzyme activity Homo sapiens
2.1.1.348 H538A the METTL3 mutation moderately reduces enzyme activity Homo sapiens
2.1.1.348 N539A the METTL3 mutation completely abolishes enzyme activity Homo sapiens
2.1.1.348 N549A the METTL3 mutation moderately reduces enzyme activity Homo sapiens
2.1.1.348 Q550A the METTL3 mutation moderately reduces enzyme activity Homo sapiens
2.1.1.348 R536A the METTL3 mutation moderately reduces enzyme activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.348 S-adenosyl-L-methionine + adenine in RNA Homo sapiens
-
 S-adenosyl-L-homocysteine + N6-methyladenine in RNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.348 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.348 Ni-NTA column chromatography, Source 15Q column chromatography, and Superdex S200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.348 additional information in the enzyme complex, METTL3 primarily functions as the catalytic core, while METTL14 serves as an RNA-binding platform Homo sapiens ?
-
-
2.1.1.348 additional information no activity with RNA probe 5-GGGCUGGGCUGGGCUGGGCU-3 Homo sapiens ?
-
-
2.1.1.348 S-adenosyl-L-methionine + 5-GGACUGGACUGGACUGGACU-3
-
 Homo sapiens S-adenosyl-L-homocysteine + 5-GGm6ACUGGm6ACUGGm6ACUGGm6ACU-3
-
 ?
2.1.1.348 S-adenosyl-L-methionine + adenine in RNA
-
 Homo sapiens S-adenosyl-L-homocysteine + N6-methyladenine in RNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.348 m6A MTase
-
 Homo sapiens
2.1.1.348 methyltransferase-like 14
-
 Homo sapiens
2.1.1.348 methyltransferase-like 3
-
 Homo sapiens
2.1.1.348 METTL14
-
 Homo sapiens
2.1.1.348 METTL3 also known as MT-A70 Homo sapiens